Molecular Cloning of a Gene Coding for a Vibrio cholerae Haemagglutinin
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چکیده
منابع مشابه
Molecular cloning and transcriptional regulation of ompT, a ToxR-repressed gene in Vibrio cholerae.
In pathogenic Vibrio cholerae, at least 17 genes are co-ordinately regulated by ToxR. Most of these genes, including those that encode cholera toxin (CT), toxin co-regulated pilus (TCP), accessory colonization factor (ACF) and OmpU, are positively regulated. OmpT is the only identified protein under negative regulation of ToxR. To understand the molecular mechanism by which ToxR represses OmpT ...
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Knowing the nucleotide sequence of the cholera toxin operon, we designed oligonucleotide primers for its-PCR amplification from local clinical isolates of V. cholerae. The resulting amplification product was cloned in a common pUC18 vector. Subsequently, a part of this operon encoding the cholera toxin Bsubunit (CTB) was reamplified and cloned between the BamH1 and EcoR1 sites of the same ...
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The structural gene for the Vibrio cholerae leucine aminopeptidase (lap) was cloned and sequenced. The cloned DNA fragment contained a 1,503-bp open reading frame potentially encoding a 501-amino-acid polypeptide with a calculated molecular mass of 54,442 Da. The deduced amino acid sequence of the entire protein showed high homology with the sequence of Vibrio proteolyticus leucine aminopeptida...
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Using antiserum to the purified soluble hemagglutinin we isolated an Escherichia coli K-12 clone expressing the gene for a hemagglutinin from Vibrio cholerae 569B. The plasmid present in this clone was designated pPM471. By deletion analysis with both specific restriction endonucleases and Bal 31 nuclease, we localized the gene, to a 0.72-kilobase region of DNA, implying a molecular weight of l...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1986
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-132-8-2225